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hydrogen and methane which is satisfactory of hydrogen and methane production yield was
achieved; (2) Source of inoculum is one of the important role of both hydrogen and methane
formations, the highest hydrogen production yield was 270 mL H 2/gCOD using
Thermoanaerobacterium-rich sludge which was 5 times greater than that achieved from the
utilized of Thermotolerant as inoculum. Meanwhile, the utilization of cow manure as inoculum
for generates methane is satisfied of methane production yield was achieved; (3) Reactor
performance, methane production yield achieved from UASB and EGSB reactors are not
different significant, despite of higher methane production yield achieved from EGSB reactor, in
contrast the UASB reactor had more stable of methane production as well as lower of VFA
accumulation; (4) Temperature, there are several advantages of thermophilic over mesophilic
temperatures are including destruction of pathogens, higher both chemical and biological
reaction rates, less variety of fermentation end-products and decreased contamination by
hydrogen consumers.