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               2.1.1.1.2 Proteins




                       Natural rubber latex collected from the Hevea trees mostly contains about 1-1.5% of total
               proteins. However, these proteins can be separated in three parts that consist of 20-27% of the
               total proteins absorbed in rubber surface, 47-66% of the total proteins in aqueous serum, and 14-
               25% of the total proteins in lutoids (Archer, 1960).



               2.1.1.1.3 Lipids and phospholipids



                       The  stability  and  colloidal  behavior  of  latex  depending  upon  lipids  and  phospholipids
               associated with rubber and non-rubber particles in latex. Lipids and phospholipids associated in
               natural rubber latex can be separated in two parts, the first part is phospholipids of the rubber
               particles are -lecithin and other lipids are a subgroup of lipids called triglycerides and waxes
               and the second part was presented in serum (Sansatsadeekul et al., 2011).


                       Some physical and chemical characteristics of skim latex serum are given in Table 2.1.
               The result shows  that there are  several  limitations of  using skim latex  serum as  substrate  for
               generate biogas such as low C/N ratio around 7 as well as relatively high inhibitants content.
               These inhibitants that contains in the skim latex serum could potentially inhibit the microbial
               growth, resulting decrease in biodegradability. Thus, to enhance the biogas production potential
               as well as to prevent the risky of the process failure, the usages of anaerobic co-digestion and a
               two-stage process is a simply practical approach as previously mentioned. The process involved
               in concentration of natural rubber latex by using centrifugation method as shown in Fig. 2.1.
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