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3) To investigate the effect of hydraulic retention time (HRT) and organic loading rate
(OLR) on hydrogen production from the optimum conditions in continuously stirred
tank (CSTR) reactor.
4) To investigate the effect of organic loading rate (OLR) on methane production in
upflow anaerobic sludge blanket (UASB) reactor.

1.3 Research scope

Scope of this study was divided into 2 parts as shown in Fig. 1.1

Optimization of hydrogen and methane production with a two-stage anaerobic co-digestion of
skim latex serum and palm oil mill effluents under thermophilic condition

Batch experiments Continuous experiments

Determination of biohydrogen production A Two-stage anaerobic process for sequential
potential (BHP) and biomethane production production of hydrogen and methane from
potential (BMP) of anaerobic dark co- thermophilic dark co-digestion of skim latex
digestion of skim latex serum and palm oil serum and palm oil mill effluent which was

mill effluent using thermophilic mixed operated in a continuously stirred tank (CSTR)
cultures under thermophilic temperature reactor and upflow anaerobic sludge blanket
(UASB) reactor
 The first stage is to produce
biohydrogen  H 2-CSTR reactor

o Mixing ratios optimization o Effect of hydraulic retention
o Optimize nutrients and buffer time (HRT)
concentrations o Effect of organic loading rate
 The second stage is to produce (OLR)

biomethane  CH 4-UASB reactor

o Methane production potential o Effect of organic loading rate
of anaerobic co-digestion of (OLR)
skim latex serum and palm oil
mill effluent

Fig. 1.1 Schematic diagram of research plan for hydrogen and methane production from
anaerobic dark co-digestion of skim latex serum and palm oil mill effluent under
thermophilic condition.