Page 70 - 048

52

from batch assay was 55:45 (%v/v) with the same concentration of buffer supplemented. The H 2-
CSTR reactor was continuously operated at the HRTs of 2.25 days and 4.50 days, respectively.
At the same time, methanogenic granular sludge used in this research was obtained from Kiang
Huat Sea Gull Trading Frozen Food Public Co, Ltd., Songkhla Province; Southern Thailand. An
840 mL of methanogenic granules were directly added into CH 4-UASB reactor as cell-mass
carriers (Kongjan et al., 2014). Afterwards, 1260 mL of the enriched methanogenic inoculum
achieved from batch reactor was fed into the CH 4-UASB reactor. Then, the rest of the reactor
active volume was filled up with BA medium supplemented with sucrose 3 g/L which was
applied for a month. After that, substrate consisting of effluent achieved from H 2-CSTR reactor
under the optimal mixing ratio of SLS to POME of 55:45 (%v/v) and BA medium at a
volumetric mixing ratio of 1:1 was fed into CH 4-UASB reactor for a month. Subsequently,
substrate for CH 4-UASB reactor was changed to effluent from H 2-CSTR reactor supplemented
with NaHCO 3 2 g/L and was continuously operated at the HRT of 18 days.

Fig. 4.1 Schematic description of lab-scale bioreactor operation for sequential production of

biohydrogen and biomethane which operated under thermophilic temperature.