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Table 3.9 shows soluble metabolite products and COD balance obtained from different
mixing ratio of SLS to POME with initial organic concentration of 7 g-VS added/L. Under this
condition the major submerged fermentation products were acetic acid (22.38±0.49 mM) butyric
acid (18.48±0.04 mM) and ethanol (2.21±0.80 mM). Results indicated that hydrogen was
generated pass through acetic acid, butyric acid and ethanol fermentations with the present of
these organic acids in fermentation broth. The biomass concentration (assumed formula
C 5H 7O 2N) used in COD balance was assumed to be 15% if the sugars degraded (Kotsopoulos et
al., 2006; Kongjan, 2010). The COD balance of individual fermentations of SLS and POME,
sucrose control and the mixture consisting of SLS and POME at the mixing ratio of 55:45 (%v/v)
were 30.33, 31.85, 16.07 and 50.33% error, respectively. The possible reason for high
measurement of degraded metabolic products error achieved from all treatment is other
metabolite products such as 1,3-propanediol (Sittijunda and Reungsang, 2012), formic acid
(Prasertsan et al., 2009) and butanol (Khamtib et al., 2011) was produced, which is not
determined.